ColE1 plasmid

QUESTION

11) You isolate a mutant form of the ColE1 plasmid with a mutant in the promoter of the rop gene. This mutation appears to prevent expression of rop. Assume the copy number of the normal plasmid is 20

11) You isolate a mutant form of the ColE1 plasmid with a mutant in the promoter of the rop gene. This mutation appears to prevent expression of rop. Assume the copy number of the normal plasmid is 20 copies per cell. What do you expect the copy number to be for the rop mutant plasmid?

a.40

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b.0 – inviable plasmid

c.20

d.10

15) Which of the following molecular events will be prevented by deletion of the UvrC gene?

a.Nicking the DNA upstream and downstream of the damaged DNA

b.Very short patch repair

c.Scanning of molecule for UV-induced DNA damage

d.Conversion of cyclobutane photodimers to thymidine monomers

e.Base excision repair